Effects of cyclophosphamide and mesna on metaphase II mouse oocyte quality
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منابع مشابه
The Impact Of Myeloperoxidase And Its Related Oxidants On Metaphase Ii Mouse Oocyte Quality
We investigated the ability of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2), hydroxyl radical (OH), and hypochlorous acid (HOCl) to overcome the defensive capacity of cumulus cells, and elucidate the mechanism through which ROS differentially deteriorate oocyte quality. Metaphase II mouse oocytes with (n=1634) and without cumulus cells (n=1633) were treated with increasing co...
متن کاملThe Impact of Myeloperoxidase and Activated Macrophages on Metaphase II Mouse Oocyte Quality
Myeloperoxidase (MPO), an abundant heme-containing enzyme present in neutrophils, monocytes, and macrophages, is produced in high levels during inflammation, and associated with poor reproductive outcomes. MPO is known to generate hypochlorous acid (HOCl), a damaging reactive oxygen species (ROS) utilizing hydrogen peroxide (H2O2) and chloride (Cl-). Here we investigate the effect of activated ...
متن کاملEffects of Leukemia Inhibitory Factor on gp130 Expression and Rate of Metaphase II Development during in vitro maturation of mouse oocyte
Background: Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. Methods: Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cul...
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این مطالعه در سال 1386-87 در آزمایشگاه و مزرعه پژوهشی دانشگاه صنعتی اصفهان به منظور تعیین مناسب ترین تیمار بذری و ارزیابی اثر پرایمینگ بر روی سه رقم گلرنگ تحت سه رژیم آبیاری انجام گرفت. برخی از مطالعات اثرات سودمند پرایمینگ بذر را بر روی گیاهان مختلف بررسی کرده اند اما در حال حاضر اطلاعات کمی در مورد خصوصیات مربوط به جوانه زنی، مراحل نموی، عملکرد و خصوصیات کمی و کیفی بذور تیمار شده ژنوتیپ های م...
the vitrification of metaphase ii mouse oocyte using ethylene glycol as cryoprotectant
introduction: the purpose of this study was to investigate the effect of vitrification procedure on the survival, fertilization, development and ultrastructure of metaphase (mii) mouse oocyte. materials and methods: female nmri mice 6-10 weeks old were superovulated using ip injection of 10 iu hmg and 10 iu hcg. ovulated oocyte were collected from the ampullary portion of the oviducts at 12-13 ...
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ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2016
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2015.12.073